New phenotypic typing scheme for group B streptococci.

New phenotypic typing scheme for group B streptococci.

AIMS To develop a new typing system for group B streptococci based on 35S-methionine-labelled protein profiles of bacterial proteins. METHODS 377 clinical isolates of group B streptococci were examined by incorporation of 35S-methionine into bacterial proteins under strict anaerobic conditions. After sodium dodecylsulphate-polyacrylamide gel electrophoresis, autoradiography was performed. The...

Typing Group a Hemolytic Streptococci

Two methods of typing group A hemolytic streptococci have been described: slide agglutination and anti-M precipitin tests. The first, developed by Griffith (1), is more extensively used. I t has the distinct advantages of requiring small amounts of serum and culture, and of a quick reaction time when all conditions are favorable. I t has certain disadvantages: The granular growth of some strain...

Growth Media for Typing Group a Streptococci.

It is frequently not possible to type group A streptococei. Only 33% of group A streptococei from Nashville children in a recent study (Quinn and Martin, Am. J. Hyg. 73:193, 1961) were typable, and there have been years when as few as 5% could be typed. This study was designed to test one possible reason for failure to type certain strains of group A streptococci, namely, inadequacy of growth m...

Phage typing scheme for group D streptococci isolated from human urogenital tract.

Streptococci of Lancefield group D were isolated from 52.2% of pharyngeal, 55.5% of urethral, 56.6% of endocervical, and 75.4% of ano-rectal specimens. Seventeen phages isolated from sewage and urogenital specimens were selected for phage typing. Four of these lysed only the strains of Streptococcus faecalis and its variety liquefaciens. Another six phages lysed only the strains of Streptococcu...

New medium for enhancing pigment production of group B streptococci.